Large Volume Sample-pretreatment Microdevice Based on Sol-gel 3d Matrix
نویسندگان
چکیده
Sample-pretreatment is of great interest for micro-total-analysis-system due to the needs of relevant sample concentration and separation techniques for efficient detection. In this study, we demonstrated a novel 3-dimensional sol-gel matrix based microdevice that can process a large volume of the sample and capture target cells with magnetic nanoparticles (MNPs)-mediated colorimetric signal. The antibody-MNP-DNA-Photocleavable linker complexes were immobilized on the sol-gel and the capture was enhanced by 3D matrix and flutter pumping scheme. The concentrated cells were released after UV irradiation, and analyzed based on MNP-mediated colorimetric detection. This novel platform suggests a simple biosensor device with sample-in-answer-out capability.
منابع مشابه
In-situ Sol-gel Modification of Pdms Electrophoretic Analytical Devices
This paper reports on a rapid new method for in-situ sol-gel deposition of silica layers in PDMS microchannels. A two-step process is used; first allowing TEOS to diffuse briefly into the channel, followed by acid catalysed hydrolysis and condensation. The silica coating results in an increased and stabilised electroosmotic flow. With this novel method there is no need for pretreatment of the m...
متن کاملA rotary microsystem for simple, rapid and automatic RNA purification.
In this work, we demonstrate a novel rotary microsystem for simple, rapid and automatic influenza viral RNA purification. The microdevice consists of a silica sol-gel matrix for RNA capture, and three reservoirs for a RNA sample (R(S)), a washing solution (R(W)) and an elution buffer (R(E)) that were connected with different dimensional microfluidic channels (120 μm for R(S), 40 μm for R(W), an...
متن کاملA microbead-incorporated centrifugal sample pretreatment microdevice.
In this study, we present a novel bead-incorporated centrifugal sample pretreatment microdevice to purify influenza A H3N2 viral RNA. Simple revolution per minute (RPM) control can lead to RNA capture on a bead-bed, and the sequential loading of a washing solution and an elution solution. Tetraethoxy orthosilicate (TEOS)-treated glass microbeads were utilized as a capture matrix. The sample pre...
متن کاملGlass-chip-based sample preparation and on-chip trypic digestion for matrix-assisted laser desorption/ionization mass spectrometric analysis using a sol-gel/2,5-dihydroxybenzoic acid hybrid matrix.
A glass-chip-based sample preparation method for matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) analysis of tryptic digests of proteins and intact cells is described. A MALDI matrix, 2,5-dihydroxybenzoic acid (2,5-DHB), was hybridized with sol-gels to generate a sol-gel-derived material. Taking advantage of the characteristics of sol-gels, the sol-gel-derived material...
متن کاملMolecularly imprinted TiO2-matrix-assisted laser desorption/ionization mass spectrometry for selectively detecting alpha-cyclodextrin.
This study describes a new means to conduct molecular recognition-based analysis using mass spectrometry. Taking advantage of the unique characteristic of the absorption capacity of the TiO(2) sol-gel material in the UV region, a TiO(2) sol-gel-deposited thin film was employed as the sample substrate to assist in UV laser desorption/ionization of analytes. Sol-gels are polymeric materials that ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2010